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Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.

机译：果蝇和哺乳动物的Polycomb组蛋白在转染的哺乳动物细胞中的转录抑制。

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The Polycomb group (Pc-G) genes are essential for maintaining the proper spatially restricted expression pattern of the homeotic loci during Drosophila development. The Pc-G proteins appear to function at target loci to maintain a state of transcriptional repression. The murine oncogene bmi-1 has significant homology to the Pc-G gene Posterior sex combs (Psc) and a highly related gene, Suppressor two of zeste [Su(z)2]. We show here that the proteins encoded by bmi-1 and the Pc-G genes Polycomb (Pc) and Psc as well as Su(z)2 mediate repression in mammalian cells when targeted to a promoter by LexA in a cotransfection system. These fusion proteins repress activator function by as much as 30-fold, and the effect on different activation domains is distinct for each Pc-G protein. Repression is observed when the LexA fusion proteins are bound directly adjacent to activator binding sites and also when bound 1,700 bases from the promoter. These data demonstrate that the products of the Pc-G genes can significantly repress activator function on transiently introduced DNA. We suggest that this function contributes to the stable repression of targeted loci during development.

机译：Polycomb组（Pc-G）基因对于在果蝇发育过程中维持顺势基因座的适当空间受限表达模式至关重要。 Pc-G蛋白似乎在目标基因座上起作用，以维持转录抑制状态。鼠癌基因bmi-1与Pc-G基因后性梳（Psc）和高度相关的基因zeste的抑制子[Su（z）2]具有显着的同源性。我们在这里显示了由bmi-1和Pc-G基因Polycomb（Pc）和Psc以及Su（z）2编码的蛋白质在共转染系统中被LexA靶向启动子时介导了哺乳动物细胞中的阻遏。这些融合蛋白将激活因子的功能抑制多达30倍，并且每种Pc-G蛋白对不同激活域的作用都不同。当LexA融合蛋白直接与激活物结合位点相邻结合时，以及与启动子结合1,700个碱基时，均观察到抑制作用。这些数据表明，Pc-G基因的产物可以显着抑制瞬时导入的DNA上的激活剂功能。我们建议此功能有助于在开发过程中稳定抑制目标基因座。

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Bunker, C A; Kingston, R E;
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年度 1994
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1. Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells. [J] . C A Bunker, R E Kingston Molecular and Cellular Biology . 1994,第3期

机译：果蝇和哺乳动物的Polycomb组蛋白在转染的哺乳动物细胞中的转录抑制。
2. Characterization of Interactions between the Mammalian Polycomb-Group Proteins Enx1/EZH2 and EED Suggests the Existence of Different Mammalian Polycomb-Group Protein Complexes [J] . Richard G. A. B. Sewalt, Johan van der Vlag, Marco J. Gunster, Molecular and Cellular Biology . 1998,第6期

机译：哺乳动物polycomb组蛋白Enx1 / EZH2和EED之间相互作用的表征表明存在不同的哺乳动物polycomb组蛋白复合物的存在。
3. Groucho/transducin-like enhancer of split (TLE) family members interact with the yeast transcriptional co-repressor SSN6 and mammalian SSN6-related proteins: implications for evolutionary conservation of transcription repression mechanisms. [J] . Grbavec D, Lo R, Liu Y, The Biochemical Journal . 1999,第Pta1期

机译：Groucho /转导蛋白样分裂增强子（TLE）家族成员与酵母转录共抑制子SSN6和哺乳动物SSN6相关蛋白相互作用：对转录抑制机制的进化保守意义。
4. Predictions of Genetic Circuit Behaviors Based on Modular Composition in Transiently Transfected Mammalian Cells [C] . Junmin Wang, Samuel A. Isaacson, Calin Belta IEEE Life Sciences Conference . 2018

机译：基于模块组成的瞬时转染哺乳动物细胞遗传电路行为的预测。
5. Transcriptional control of the mammalian Unfolded Protein Response: Roles of XBP1 in non-secretory and secretory cells. [D] . Acosta-Alvear, Diego. 2008

机译：哺乳动物未折叠蛋白应答的转录控制：XBP1在非分泌和分泌细胞中的作用。
6. Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells. [O] . C A Bunker, R E Kingston 1994

机译：果蝇和哺乳动物的Polycomb组蛋白在转染的哺乳动物细胞中的转录抑制。
7. Transcriptional repression by Drosophila and mammalian polycomb group proteins in transfected mammalian cells [O] . C A Bunker, R E Kingston, Christopher A. Bunker, 1994

机译：果蝇和哺乳动物的多梳组蛋白在转染的哺乳动物细胞中的转录抑制。

Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.

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